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Index one: the i7 index column is in the ahead orientation (match the orientation Should the i7 indexes within the sample sheet).
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When demultiplexing, the ultimate sample sheet delivered to your FASTQ generation software package must hold the i5 index in the correct orientation for that System. However, most Illumina sample sheet technology software package handles this instantly.
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When examining index sets for shade stability, the sequence observed by the instrument needs to be assessed, whatever the orientation utilized for the sample sheet. For the i7 index, always asses shade stability utilizing the "i7 bases for sample sheet" column orientation.
The sequences shown are precisely what is entered into the final sample sheet. Kit definitions possess the i5 inside the forward orientation. The i5 sequences will be correctly reverse complemented and exhibited from the IEM interface based upon instrument/reagent form.